vegetОвощеводствоVegetable Growing0201-8411Институт овощеводстваveget-115Research ArticleСтатьиИСПОЛЬЗОВАНИЕ ПРОРОСТКОВ СЕМЯН И ДНК-МАРКЕРОВ ДЛЯ ОЦЕНКИ ОБРАЗЦОВ ФАСОЛИ ОБЫКНОВЕННОЙ (PHASEOLUS VULGARIS L.) ИЗ КОЛЛЕКЦИИ ВИР ПО УСТОЙЧИВОСТИ К АНТРАКНОЗУUSE OF SEEDLINGS AND DNA MARKERS FOR EVALUATION OF GARDEN BEAN (PHASEOLUS VULGARIS L.) SAMPLES FROM THE VIR-COLLECTION FOR ANTHRACNOSE RESISTANCEЮйтинСяоYutingXiao

Аспирант

г. Минск

СаукИ. Б.SaukI. B.

Старший научный сотрудник

г. Минск

АнохинаВ. С.AnohinaV. S.

Кандидат биологических наук, доцент кафедры генетики, заведующий сектором генетики растений НИЛ молекулярной генетики и биотехнологии

г. Минск

Белорусский государственный университетBelarus202027042022280185197Copyright © Юйтин С., Саук И.Б., Анохина В.С., 20222022Юйтин С., Саук И.Б., Анохина В.С.Yuting X., Sauk I.B., Anohina V.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://veget.belal.by/jour/article/view/115

Представлены результаты изучения различающихся по происхождению сортов фасоли обыкновенной (Phaseolus vulgaris L.) по устойчивости к антракнозу с использованием проростков и молекулярного тестирования по генам устойчивости (Co-14, Co-2,, Co-4, Co-6). Для анализа проростков использовали рулонный метод и чашки Петри. Наиболее достоверные данные получены с использованием чашек Петри. На основе лабораторных анализов проростков по устойчивости к антракнозу и ДНК-маркерам выделены перспективные образцы фасоли (Сакса без волокна 615 и Лаура).

The research results of garden bean samples (Phaseolus vulgaris L.) to anthracnose resistance using seedlings and molecular testing for the Co-14, Co-2, Co-4, Co-6 genes were presented. Roll method and Petri dishes were used for the seedlings analysis. The most reliable data were obtained using Petri dishes. Promising bean samples (Saksa bez volokna 615 and Laura) were identified based on seedlings analyzes for anthracnose resistance and DNA-markers.

антракнозгенотипДНК-маркерпроростокфасоль обыкновеннаяanthracnosegenotypeDNA-markerseedlinggarden beanReferencesFAO [Electronic resourсe]. – Mode of access: http: // faostat.fao.org. – Date of access: 06.08.2020.FAO [Electronic resourсe]. – Mode of access: http: // faostat.fao.org. – Date of access: 06.08.2020.Фасоль спаржевая в Беларуси / А. И. Чайковский [и др.]. – Минск : Типография ВЮА, 2009. – 168 с.Фасоль спаржевая в Беларуси / А. И. Чайковский [и др.]. – Минск : Типография ВЮА, 2009. – 168 с.Исходный материал для селекции овощных зернобобовых культур в коллекции ВИР / М. А. Вишнякова [и др.] // Овощи России. – 2013. – № 1. – С. 16–25.Исходный материал для селекции овощных зернобобовых культур в коллекции ВИР / М. А. Вишнякова [и др.] // Овощи России. – 2013. – № 1. – С. 16–25.Genetic Mapping and QTL Analysis in Common Bean / A. M. Gonzalez [et al.] // The Common Bean Genome / М. Perez de la Vega, М. Santalla, F. Marsolais (eds.). – Springer, Cham. – 2017. – P. 69–107.Genetic Mapping and QTL Analysis in Common Bean / A. M. Gonzalez [et al.] // The Common Bean Genome / М. Perez de la Vega, М. Santalla, F. Marsolais (eds.). – Springer, Cham. – 2017. – P. 69–107.Genetics and mapping of a new anthracnose resistance locus in Andean common bean Paloma / S. A. de Lima Castro [et al.] // BMC Genomics. – 2017. – Vol. 18. – P. 306–317.Genetics and mapping of a new anthracnose resistance locus in Andean common bean Paloma / S. A. de Lima Castro [et al.] // BMC Genomics. – 2017. – Vol. 18. – P. 306–317.Barrus, M. F. An anthracnose-resistant red kidney bean / M. F. Barrus // Phytopathology. – 1915. – Vol. 5. – P. 303–311.Barrus, M. F. An anthracnose-resistant red kidney bean / M. F. Barrus // Phytopathology. – 1915. – Vol. 5. – P. 303–311.Melotto, M. An allelic series at the Co-1 locus conditioning resistance to anthracnose in common bean of Andean origin / M. Melotto, J. D. Kelly // Euphytica. – 2000. – Vol. 116. – P. 143–149.Melotto, M. An allelic series at the Co-1 locus conditioning resistance to anthracnose in common bean of Andean origin / M. Melotto, J. D. Kelly // Euphytica. – 2000. – Vol. 116. – P. 143–149.Allelism studies for anthracnose resistance genes of common bean cultivar AND 277 / Alzate-Marin [et al.] // Annu. Rpt. Bean. Improv. Coop. – 2003. – Vol. 46. – P. 173–174.Allelism studies for anthracnose resistance genes of common bean cultivar AND 277 / Alzate-Marin [et al.] // Annu. Rpt. Bean. Improv. Coop. – 2003. – Vol. 46. – P. 173–174.Linkage mapping of the Phg-1 and Co-14 genes for resistance to angular leaf spot and anthracnose in the common bean cultivar AND277 / M. C. Goncalves- Vidigal [et al.] // Theor. Appl. Genet. – 2011. – Vol. 122. – P. 893–903.Linkage mapping of the Phg-1 and Co-14 genes for resistance to angular leaf spot and anthracnose in the common bean cultivar AND277 / M. C. Goncalves- Vidigal [et al.] // Theor. Appl. Genet. – 2011. – Vol. 122. – P. 893–903.Mastenbrock, C. A breeding programе for resistance to anthracnose in dry shell haricot beans, based on a new gene / C. Mastenbrock // Euphytica. – 1960. – Vol. 9. – P. 177–184.Mastenbrock, C. A breeding programе for resistance to anthracnose in dry shell haricot beans, based on a new gene / C. Mastenbrock // Euphytica. – 1960. – Vol. 9. – P. 177–184.Tu, J. C. Colletotrichum lindemuthianum on bean. Population dynamics of the pathogen and breeding for resistance / J. C. Tu // Colletotrichum: Biology, pathology and control / J. A. Bailey, M. J. Jeger (eds.). – CAB Intl., Wallingford, U. K. – 1992. – P. 203–224.Tu, J. C. Colletotrichum lindemuthianum on bean. Population dynamics of the pathogen and breeding for resistance / J. C. Tu // Colletotrichum: Biology, pathology and control / J. A. Bailey, M. J. Jeger (eds.). – CAB Intl., Wallingford, U. K. – 1992. – P. 203–224.Kelly, J. D. A comprehensive review of the major genes conditioning resistance to anthracnose in common bean / J. D. Kelly, V. A. Vallejo // HortScience. – 2004. – Vol. 39, № 6. – P. 1191–1207.Kelly, J. D. A comprehensive review of the major genes conditioning resistance to anthracnose in common bean / J. D. Kelly, V. A. Vallejo // HortScience. – 2004. – Vol. 39, № 6. – P. 1191–1207.SCAR, RAPD and RFLP markers linked to a dominant gene (Are) conferring resistance to anthracnose / A. F. Adam-Blondon [et al.] // Theor. Appl. Genet. – 1994. – Vol. 88. – P. 865–870.SCAR, RAPD and RFLP markers linked to a dominant gene (Are) conferring resistance to anthracnose / A. F. Adam-Blondon [et al.] // Theor. Appl. Genet. – 1994. – Vol. 88. – P. 865–870.Fouilloux, G. Bean anthracnose: New genes of resistance / G. Fouilloux // Annu. Rpt. Bean Improv. Coop. – 1976. – Vol. 19. – P. 36–37.Fouilloux, G. Bean anthracnose: New genes of resistance / G. Fouilloux // Annu. Rpt. Bean Improv. Coop. – 1976. – Vol. 19. – P. 36–37.Marker – assisted dissection of the oligogenic anthracnose resistance in the common bean cultivar “G2333”/ R. A. Yong [et al.] // Theor. Appl. Genet. – 1998. – Vol. 96. – P. 87–94.Marker – assisted dissection of the oligogenic anthracnose resistance in the common bean cultivar “G2333”/ R. A. Yong [et al.] // Theor. Appl. Genet. – 1998. – Vol. 96. – P. 87–94.Balardin, R. S. Virulence and molecular diversity in Colletotrichum lindemuthianum from South, Central and North America / R. S. Balardin, A. Jarosz, J. D. Kelly // Phytopathology. – 1997. – Vol. 87. – P. 1184–1191.Balardin, R. S. Virulence and molecular diversity in Colletotrichum lindemuthianum from South, Central and North America / R. S. Balardin, A. Jarosz, J. D. Kelly // Phytopathology. – 1997. – Vol. 87. – P. 1184–1191.Balardin, R. S. Interaction among races of Colletotrichum lindemuthianum and diversity in Phaseolus vulgaris / R. S. Balardin, J. D. Kelly // J. Amer. Soc. Hort. Sci. – 1998. – Vol. 123. – P. 1038–1047.Balardin, R. S. Interaction among races of Colletotrichum lindemuthianum and diversity in Phaseolus vulgaris / R. S. Balardin, J. D. Kelly // J. Amer. Soc. Hort. Sci. – 1998. – Vol. 123. – P. 1038–1047.Young, R. A. Characterization of the genetic resistance to Colletotrichum lindemuthianum in common bean differential cultivars / R. A. Young, J. D. Kelly // Plant Dis. – 1996. – Vol. 80. – P. 650–654.Young, R. A. Characterization of the genetic resistance to Colletotrichum lindemuthianum in common bean differential cultivars / R. A. Young, J. D. Kelly // Plant Dis. – 1996. – Vol. 80. – P. 650–654.Inheritance of anthracnose resistance in common bean genotypes PI 207262 and AB 136 / M. C. Goncalves-Vidigal [et al.] // Brazilian J. Genetics. – 1997. – Vol. 20. – P. 59–62.Inheritance of anthracnose resistance in common bean genotypes PI 207262 and AB 136 / M. C. Goncalves-Vidigal [et al.] // Brazilian J. Genetics. – 1997. – Vol. 20. – P. 59–62.Menezes, J. R. Race characterization of Brazilian isolates of Colletotrichum lindemuthianum and detection of resistance to anthracnose in Phaseolus vulgaris / J. R. Menezes, J. C. Dianese // Phytopathology. – 1988. – Vol. 78. – P. 650–655.Menezes, J. R. Race characterization of Brazilian isolates of Colletotrichum lindemuthianum and detection of resistance to anthracnose in Phaseolus vulgaris / J. R. Menezes, J. C. Dianese // Phytopathology. – 1988. – Vol. 78. – P. 650–655.Application of Common Bean Anthracnose Resistance Gene SCAR Markers in Snap Bean Disease Resistance Identification / GU Yu [et al.] // Acta Horticulturae Sinica. – 2011. – Vol. 38, № 5. – P. 911–920.Application of Common Bean Anthracnose Resistance Gene SCAR Markers in Snap Bean Disease Resistance Identification / GU Yu [et al.] // Acta Horticulturae Sinica. – 2011. – Vol. 38, № 5. – P. 911–920.Mapping of Gene Conferring Resistance to Anthracnose in Common Bean (Phaseolus vulgaris L.) by Molecular Markers / Ming-Li CHEN [et al.] // Acta Agronomica Sinica. – 2011. – Vol. 37, № 12. – P. 2130–2135.Mapping of Gene Conferring Resistance to Anthracnose in Common Bean (Phaseolus vulgaris L.) by Molecular Markers / Ming-Li CHEN [et al.] // Acta Agronomica Sinica. – 2011. – Vol. 37, № 12. – P. 2130–2135.NIE, Chu-chu. Research Progress of Kidney Bean Breeding in China / Chu-chu NIE, Yu-zhen HAN // Journal of Changjiang Vegetables. – 2011. – Vol. 2. – P. 1–5.NIE, Chu-chu. Research Progress of Kidney Bean Breeding in China / Chu-chu NIE, Yu-zhen HAN // Journal of Changjiang Vegetables. – 2011. – Vol. 2. – P. 1–5.Mapping and genetic structure analysis of anthracnose resistance locus Co-1HY in the Common Bean (Phaseolus vulgaris L.) / M. Chen [et al.] // PLoS One 12(1):e016954 doi:10.1371/Journal.pone.0169954.Mapping and genetic structure analysis of anthracnose resistance locus Co-1HY in the Common Bean (Phaseolus vulgaris L.) / M. Chen [et al.] // PLoS One 12(1):e016954 doi:10.1371/Journal.pone.0169954.Якушева, А. С. Оценка люпина на устойчивость к антракнозу : метод. рекомендации / А. С. Якушева, Н. Н. Соловьянова. – Брянск, 2001. – 18 с.Якушева, А. С. Оценка люпина на устойчивость к антракнозу : метод. рекомендации / А. С. Якушева, Н. Н. Соловьянова. – Брянск, 2001. – 18 с.Семена сельскохозяйственных культур. Методы определения всхожести : ГОСТ 12038-84. – Взамен ГОСТ 12038-66М ; утв. и введ. в действие постановлением Гос. ком. СССР по стандартам от 19.12.84 г. № 4710. – М. : Стандартинформ, 2011. – 64 с.Семена сельскохозяйственных культур. Методы определения всхожести : ГОСТ 12038-84. – Взамен ГОСТ 12038-66М ; утв. и введ. в действие постановлением Гос. ком. СССР по стандартам от 19.12.84 г. № 4710. – М. : Стандартинформ, 2011. – 64 с.Широкий унифицированный классификатор СЭВ и международный классификатор СЭВ культурных видов рода Phaseolus L. / Науч.-техн. совет стран – членов СЭВ по кол. диких и культур. видов растений и др. – Л. : ВИР, 1984. – 37 с.Широкий унифицированный классификатор СЭВ и международный классификатор СЭВ культурных видов рода Phaseolus L. / Науч.-техн. совет стран – членов СЭВ по кол. диких и культур. видов растений и др. – Л. : ВИР, 1984. – 37 с.SQ4 SCAR marker linked to the Co-2 gene on B11 appears to be linked to the Ur 11f gene / H. S. Awale [et al.] // Annu. Rept. Bean Improv. Coop. – 2008. – Vol. 51. – P. 174–175.SQ4 SCAR marker linked to the Co-2 gene on B11 appears to be linked to the Ur 11f gene / H. S. Awale [et al.] // Annu. Rept. Bean Improv. Coop. – 2008. – Vol. 51. – P. 174–175.Development of SCAR markers linked to common bean anthracnose resistance genes Co-4 and Co-6 / V. T. Queiroz [et al.] // Annu. Rep. Bean Improv. Coop. – 2004. – Vol. 47. – P. 249–250.Development of SCAR markers linked to common bean anthracnose resistance genes Co-4 and Co-6 / V. T. Queiroz [et al.] // Annu. Rep. Bean Improv. Coop. – 2004. – Vol. 47. – P. 249–250.Awale, H. E. Development of SCAR markers linked to Co-42 gene in common bean / H. E. Awale, J. D. Kelly // Annu. Report. Bean Improv. Coop. – 2001. – Vol. 44. – P. 119–120.Awale, H. E. Development of SCAR markers linked to Co-42 gene in common bean / H. E. Awale, J. D. Kelly // Annu. Report. Bean Improv. Coop. – 2001. – Vol. 44. – P. 119–120.

The authors declare that there are no conflicts of interest present.